High-performance thin-layer chromatography

Not to be confused with HPLC.

High-performance thin-layer chromatography (HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds.[1] This TLC-based analytical technique enhances compound resolution for quantitative analysis. Some of these improvements involve employing higher-quality TLC plates with finer particle sizes in the stationary phase, leading to improved resolution.[2] Additionally, the separation can be further refined through repeated plate development using a multiple development device. As a result, HPTLC provides superior resolution and lower Limit of Detection (LODs).[3]

  1. ^ Morlock, Gertrud E. (2 October 2021). "High-performance thin-layer chromatography combined with effect-directed assays and high-resolution mass spectrometry as an emerging hyphenated technology: A tutorial review". Analytica Chimica Acta. 1180: 338644. doi:10.1016/j.aca.2021.338644. ISSN 0003-2670. PMID 34538319. S2CID 236348479.
  2. ^ Kaiser, R. E. (September 1978). "Anticircular high performance thin‐layer chromatography". Journal of High Resolution Chromatography. 1 (3): 164–168. doi:10.1002/jhrc.1240010309. ISSN 0935-6304.
  3. ^ Le Roux, A.M.; Wium, C.A.; Joubert, J.R.; Van Jaarsveld, P.P. (October 1992). "Evaluation of a high-performance thin-layer chromatographic technique for the determination of salbutamol serum levels in clinical trials". Journal of Chromatography B: Biomedical Sciences and Applications. 581 (2): 306–309. doi:10.1016/0378-4347(92)80288-2. ISSN 0378-4347. PMID 1452625.
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