Hydrophilic interaction chromatography

See also: Chromatography

Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC)[1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Andrew Alpert in his 1990 paper on the subject.[2] He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re-evaluation of published data.[3]

  1. ^ Jandera, Pavel (2011). "Stationary and mobile phases in hydrophilic interaction chromatography: a review". Analytica Chimica Acta. 692 (1): 1–25. Bibcode:2011AcAC..692....1J. doi:10.1016/j.aca.2011.02.047. ISSN 0003-2670. PMID 21501708.
  2. ^ Alpert, Andrew J. (1990). "Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds". Journal of Chromatography. 499: 177–196. doi:10.1016/S0021-9673(00)96972-3. PMID 2324207.
  3. ^ Petrus Hemström and Knut Irgum (2006). "Review: Hydrophilic Interaction Chromatography". J. Sep. Sci. 29 (12): 1784–1821. doi:10.1002/jssc.200600199. PMID 16970185.
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