QPNC-PAGE

QPNC-PAGE, or Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis, is a bioanalytical, high-resolution and high-precision zone electrophoresis technique applied in biochemistry and bioinorganic chemistry to separate proteins or protein isoforms by isoelectric point and by continuous elution from a gel column for further characterization.[1][2]

This standardized 1-D hybrid variant of native gel electrophoresis and preparative polyacrylamide gel electrophoresis is used to quantitatively resolve physiological concentrations of macromolecules with high recovery, for example, into active or native metalloproteins in biological samples or into properly and improperly folded metal cofactor-containing proteins in complex protein mixtures.[1][3]

  1. ^ a b Michov, B. (2022). Electrophoresis Fundamentals: Essential Theory and Practice. De Gruyter, ISBN 9783110761627. doi:10.1515/9783110761641. ISBN 9783110761641.
  2. ^ Seelert H, Krause F (2008). "Preparative isolation of protein complexes and other bioparticles by elution from polyacrylamide gels". Electrophoresis. 29 (12): 2617–36. doi:10.1002/elps.200800061. PMID 18494038. S2CID 35874355.
  3. ^ Kastenholz B (2007). "New hope for the diagnosis and therapy of Alzheimer's disease". Protein and Peptide Letters. 14 (4): 389–93. doi:10.2174/092986607780363970. PMID 17504097.
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