Salting out

Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.[1] Salting out is typically used to precipitate large biomolecules, such as proteins or DNA.[2] Because the salt concentration needed for a given protein to precipitate out of the solution differs from protein to protein, a specific salt concentration can be used to precipitate a target protein. This process is also used to concentrate dilute solutions of proteins. Dialysis can be used to remove the salt if needed.

  1. ^ Genck, Wayne (7 November 2010). "Make The Most of Antisolvent Crystallization". Chemical Processing. Putman Media. Retrieved 12 June 2025.
  2. ^ Chacon-Cortes, D; Griffiths, L (4 December 2020). "Methods for extracting genomic DNA from whole blood samples: current perspectives". Journal of Biorepository Science for Applied Medicine. 2014 (2): 1–9. doi:10.2147/BSAM.S46573.
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