Auramine phenol stain

Auramine phenol stain is a stain used in clinical microbiology and histology to identify tuberculosis mycobacteria.

There are two types of auramine phenol stains, 1 and 2 to stain mycobacterium species and cryptosporidium respectively. Both are fluorescent stains. The bacteria or the parasites appear brilliant greenish yellow against dark background.[1] Mycolic acids of the mycobacteria keep this stain when decolorising with the acid alcohol. The method is more rapid and sensitive than ZN technique.[2]

  1. ^ "Microbiology training log". Retrieved 19 November 2012.
  2. ^ "Comparison of the value of two different sputum staining for diagnosis of acid-fast bacilli". Retrieved 19 November 2012.
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