Intravital microscopy

Intravital microscopy is a form of microscopy that allows observing biological processes in live animals (in vivo) at a high resolution that makes distinguishing between individual cells of a tissue possible.[1]

In mammals, in some experimental settings a surgical implantation of an imaging window is performed prior to intravital microscopy. This allows repeated observations over several days or weeks. For example, if researchers want to visualize liver cells of a live mouse they will implant an imaging window into mouse's abdomen.[2] Mice are the most common choice of animals for intravital microscopy but in special cases other rodents such as rats might be more suitable. Animals are usually anesthetized throughout surgeries and imaging sessions. Intravital microscopy is used in several areas of research including neurology, immunology, stem cell studies and others. This technique is particularly useful to assess a progression of a disease or an effect of a drug.[1]

  1. ^ a b Masedunskas, Andrius; Milberg, Oleg; Porat-Shliom, Natalie; Sramkova, Monika; Wigand, Tim; Amornphimoltham, Panomwat; Weigert, Roberto (2012). "Intravital microscopy A practical guide on imaging intracellular structures in live animals". Bioarchitecture. 2 (5): 143–157. doi:10.4161/bioa.21758. PMC 3696059. PMID 22992750.
  2. ^ Ritsma, Laila; Steller, Ernst J. A.; Beerling, Evelyne; Loomans, Cindy J. M.; Zomer, Anoek; Gerlach, Carmen; Vrisekoop, Nienke; Seinstra, Daniëlle; Gurp, Leon van; Schäfer, Ronny; Raats, Daniëlle A.; Graaff, Anko de; Schumacher, Ton N.; Koning, Eelco J. P. de; Rinkes, Inne H. Borel; Kranenburg, Onno; Rheenen, Jacco van (31 October 2012). "Intravital Microscopy Through an Abdominal Imaging Window Reveals a Pre-Micrometastasis Stage During Liver Metastasis". Science Translational Medicine. 4 (158): 158ra145. doi:10.1126/scitranslmed.3004394. ISSN 1946-6234. PMID 23115354. S2CID 20938420.
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