Methanosarcina barkeri
| Methanosarcina barkeri | |
|---|---|
| Phase-contrast photo of Methanosarcina barkeri, type strain MST | |
| Scientific classification | |
| Domain: | Archaea |
| Kingdom: | Methanobacteriati |
| Phylum: | Methanobacteriota |
| Class: | "Methanomicrobia" |
| Order: | Methanosarcinales |
| Family: | Methanosarcinaceae |
| Genus: | Methanosarcina |
| Species: | M. barkeri
|
| Binomial name | |
| Methanosarcina barkeri Schnellen 1947[1]
| |
Methanosarcina barkeri is the type species of the genus Methanosarcina, characterized by its wide range of substrates used in methanogenesis. While most known methanogens produce methane from H2 and CO2, M. barkeri can also dismutate methylated compounds such as methanol or methylamines, oxidize acetate, and reduce methylated compounds with H2. This makes M. barkeri one of the few Methanosarcina species capable of utilizing all four known methanogenesis pathways.[2] Even among other Methanosarcinales, which commonly utilize a broad range of substrates, the ability to grow on H2 and CO2 is rare due to the requirement for high H2 partial pressure.[3][4] Like other Methanosarcina species, M. barkeri has a large genome (4.53 Mbp for the type strain MS, 4.9 Mbp for the Wiesmoor strain, and 4.5 Mbp for the CM2 strain), although it is significantly smaller than the largest archaeal genome of Methanosarcina acetivorans (5.75 Mbp for the type strain C2A).[5][6][7] It is also one of the few archaea, particularly among anaerobic species, that is genetically tractable and can be used for genetic studies.[8][9][10]
- ^ Methanosarcina barkeri was named by Charles Gerardus Theodorus Petrus Schnellen in honor of H. Albert Barker. — Schnellen, C. G. T. P. Onderzoekingen over de methaangisting. Doctoral thesis. Tech. Univ. Delft Rotterdam, Netherlands, 1947.
- ^ Mand, Thomas D.; Metcalf, William W. (20 November 2019). "Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMethanosarcina". Microbiology and Molecular Biology Reviews. 83 (4). doi:10.1128/mmbr.00020-19. ISSN 1092-2172. PMC 6759668. PMID 31533962.
- ^ Thauer, Rudolf K.; Kaster, Anne-Kristin; Seedorf, Henning; Buckel, Wolfgang; Hedderich, Reiner (30 June 2008). "Methanogenic archaea: ecologically relevant differences in energy conservation". Nature Reviews Microbiology. 6 (8): 579–591. doi:10.1038/nrmicro1931. ISSN 1740-1526.
- ^ Feldewert, Christopher; Lang, Kristina; Brune, Andreas (21 August 2020). "The hydrogen threshold of obligately methyl-reducing methanogens". FEMS Microbiology Letters. 367 (17). doi:10.1093/femsle/fnaa137. ISSN 1574-6968. PMC 7485788. PMID 32821944.
- ^ Maeder, Dennis L.; Anderson, Iain; Brettin, Thomas S.; Bruce, David C.; Gilna, Paul; Han, Cliff S.; Lapidus, Alla; Metcalf, William W.; Saunders, Elizabeth; Tapia, Roxanne; Sowers, Kevin R. (15 November 2006). "The Methanosarcina barkeri Genome: Comparative Analysis with Methanosarcina acetivorans and Methanosarcina mazei Reveals Extensive Rearrangement within Methanosarcinal Genomes". Journal of Bacteriology. 188 (22): 7922–7931. doi:10.1128/jb.00810-06. ISSN 0021-9193. PMC 1636319. PMID 16980466.
- ^ DasSarma, Shiladitya (28 May 2002). "Faculty Opinions recommendation of The genome of M. acetivorans reveals extensive metabolic and physiological diversity". doi:10.3410/f.1006490.81806.
{{cite web}}: Missing or empty|url=(help) - ^ Protasov, Evgenii; Reeh, Hanna; Liu, Pengfei; Poehlein, Anja; Platt, Katja; Heimerl, Thomas; Hervé, Vincent; Daniel, Rolf; Brune, Andreas (6 August 2024). "Genome reduction in novel, obligately methyl-reducing Methanosarcinales isolated from arthropod guts (Methanolapillus gen. nov. and Methanimicrococcus)". FEMS Microbiology Ecology. 100 (9). doi:10.1093/femsec/fiae111. ISSN 1574-6941. PMC 11362671.
- ^ Gonnerman, Matthew C.; Benedict, Matthew N.; Feist, Adam M.; Metcalf, William W.; Price, Nathan D. (26 March 2013). "Genomically and biochemically accurate metabolic reconstruction of Methanosarcina barkeri Fusaro, iMG746". Biotechnology Journal. 8 (9): 1070–1079. doi:10.1002/biot.201200266. ISSN 1860-6768. PMID 23420771.
- ^ Metcalf, William W.; Zhang, Jun Kai; Apolinario, Ethel; Sowers, Kevin R.; Wolfe, Ralph S. (18 March 1997). "A genetic system for Archaea of the genus Methanosarcina : Liposome-mediated transformation and construction of shuttle vectors". Proceedings of the National Academy of Sciences. 94 (6): 2626–2631. doi:10.1073/pnas.94.6.2626. ISSN 0027-8424. PMC 20139. PMID 9122246.
- ^ Boccazzi, Paolo; Zhang, Jun Kai; Metcalf, William W. (May 2000). "Generation of Dominant Selectable Markers for Resistance to Pseudomonic Acid by Cloning and Mutagenesis of the ileS Gene from the Archaeon Methanosarcina barkeri Fusaro". Journal of Bacteriology. 182 (9): 2611–2618. doi:10.1128/jb.182.9.2611-2618.2000. ISSN 0021-9193. PMC 111328. PMID 10762266.