Protein misfolding cyclic amplification

Protein misfolding cyclic amplification (PMCA) is an amplification technique (conceptually like polymerase chain reaction (PCR) but not involving nucleotides) to multiply misfolded prions originally developed by Soto and colleagues.[1] It is a test for spongiform encephalopathies like chronic wasting disease (CWD)[2] or bovine spongiform encephalopathy (BSE).

  1. ^ Saborio, G.P., Permanne, B. and Soto, C. (2001) Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding. Nature, 411, 810-813.
  2. ^ Patrice N Klein, CWD Program Manager USDA/APHIS. "Chronic Wasting Disease - APHIS Proposed Rule to Align BSE Import Regulations to OIE" (PDF). WHHCC Meeting – 5–6 February 2013. Archived from the original (PDF) on 26 September 2014.{{cite web}}: CS1 maint: location (link)
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