Streaking (microbiology)

In microbiology, streaking is a mechanical technique used to isolate a pure strain from a single species of microorganism, often bacteria.[1] Samples from a colony derived from a single cell are taken from the streaked plate to create a genetically identical microbiological culture grown on a new plate so that the organism can be identified, studied, or tested.[2] Different patterns can be used to streak a plate. All involve the dilution of bacteria by systematically streaking them over the exterior of the agar in a Petri dish to obtain isolated colonies which contain gradually fewer numbers of cells.[1] If the agar surface grows microorganisms which are all genetically same, the culture is then considered as a pure microbiological culture.

  1. ^ a b Sanders, E. R. (2025-01-22). "Aseptic Laboratory Techniques: Plating Methods - PMC". Journal of Visualized Experiments (63): 3064. doi:10.3791/3064. PMC 4846335. PMID 22617405.
  2. ^ Hildebrand, E. M. (1938). "Techniques for the Isolation of Single Microorganisms". Botanical Review. 4 (12): 627–664. Bibcode:1938BotRv...4..627H. doi:10.1007/BF02869844. ISSN 0006-8101. JSTOR 4353196.